Extended storage of whole blood with 3-deazaadenosine for homocysteine assay
نویسندگان
چکیده
منابع مشابه
3-Deazaadenosine, a Stabilizer of Whole-Blood Homocysteine Content, Does Not Interfere with the Single- Enzyme Homocysteine Assay while Totally Inhibiting the Enzyme Conversion Homocysteine Immunoassay
eclampsia (at 34 weeks of gestation) had sFlt1 values within the reference interval. This woman had abruptio placenta at delivery (36 weeks of gestation). In the remaining seven patients, the sFlt1 assay would have predicted preeclampsia a mean of 11.2 weeks (range, 6.5–16.5 weeks) before clinical onset. No significant difference was observed in mean (SD) serum creatinine concentrations ( mol/L...
متن کاملAddition of 3-deazaadenosine to vacutainer tubes stabilizes whole-blood homocysteine for at least 6 hours at ambient temperature.
To the Editor: Increased plasma homocysteine is now well established as a risk factor for cardiac, cerebral, and peripheral vascular disease (1). Despite this, the test is not widely available. This is partly because of practical difficulties in sample collection. The homocys-teine concentration increases in whole blood by up to 10% per hour unless samples are kept on ice and separated within 6...
متن کاملEffects of temperature on stability of blood homocysteine in collection tubes containing 3-deazaadenosine.
BACKGROUND The accuracy of homocysteine (Hcy) results is currently compromised by the requirement to separate the plasma within 1 h of sample collection. We studied the effect of temperature on the stability of plasma Hcy over a 72-h time course in blood collected into evacuated tubes containing either EDTA alone or both EDTA and 3-deazaadenosine (3DA). METHODS We recruited 100 volunteers, in...
متن کاملCompatibility of the Abbott IMx homocysteine assay with citrate-anticoagulated plasma and stability of homocysteine in citrated whole blood.
ment. We saw no systematic error associated with a plot of the residuals (data not shown). The linear model accounted for the data, and the underlying assumptions (linearity, stable variance) of the model have not been violated. The upper limit of linearity was 3.377 mmol/L (700 mg/L), although we routinely used a linear calibration up to 1.930 mmol/L (400 mg/L), which encompassed almost all of...
متن کاملAcidic citrate stabilizes blood samples for assay of total homocysteine.
min were as follows: At 70 MPa (10 000 lb./in.), no pressure effect was evident; ;10% of maximal binding was observed at 140 MPa, 20% at 210 MPa, 50% at 280 MPa, and 80% at 420 MPa. Preincubation of the anti-p24 antibody alone (without the p24 antigen) at 420 MPa for 10 min did not result in any discernible decrease in absorbance, indicating that pressures as great as 420 MPa did not affect the...
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 2007
ISSN: 0004-5632,1758-1001
DOI: 10.1258/000456307780945732